Test Principle ImmunoCAP Total IgE

The technology is based on an extremely high total binding capacity, achieved through a high binding capacity per mg cellulose combined with an optimal quantity of cellulose in each solid phase. This ensures binding of all relevant antibodies, regardless of antibody affinity, while still giving low non-specific binding.

The ImmunoCAP solid phase consists of a cellulose derivative enclosed in a capsule. The hydrophilic, highly branched polymer provides an ideal microenvironment for allergens, binding them irreversibly while maintaining their native structure.

The test is designed as a sandwich immunoassay.

 
Anti-IgE, covalently coupled to the solid phase, reacts with the total IgE in the patient serum sample.
After washing, enzyme-labelled antibodies against IgE are added to form a complex.
After incubation, unbound enzyme-labelled anti-IgE is washed away, and the bound complex is then incubated with a developing agent.
After the reaction has been stopped, the fluorescence of the eluate is measured. The fluorescence is directly proportional to the concentration of IgE in the serum sample.