ImmunoCAP ISAC is a two-step assay:
- IgE antibodies from the patient sample bind to the immobilized allergen components.
- Allergen-bound IgE antibodies are detected by a fluorescence-labelled anti-IgE antibody.
The test procedure (including washing and incubation steps) gives a total assay time of less than 4 hours.
Fluorescence is measured with a laser scanner, and the results are evaluated using Phadia Microarray Image Analysis (MIA) software. The MIA software has a user-friendly interface and enables automatic readout and customized result reports.
ImmunoCAP ISAC is a semi-quantitative test, and results are reported in ISAC Standardized Units (ISU) giving indications of specific IgE antibody levels within a measuring range of 0.3-100 ISU-E. The ISU-E are standardized to ImmunoCAP Specific IgE units.