Test Principle ImmunoCAP ECP

The ImmunoCAP ECP assay is based upon the proven ImmunoCAP cellulose solid phase.  The ImmunoCAP solid phase consists of a three dimensional cellulose derivative, encased in a capsule, allowing for discrete, automated assay processing.  This classic “sandwich” immunoassay, by utilizing the cellulose solid phase, allows for the covalent binding of large amounts of anti-ECP capture antibodies.  The cellulose matrix also makes it possible for the instrument to perform a thorough washing between sample and reagent additions, thus accounting for the observed low background signals.

 
Anti ECP, covalently coupled to the solid phase, reacts with the ECP in the patient serum sample.
After washing, enzyme-labelled antibodies against ECP are added to form a complex.
After incubation, unbound enzyme-anti-ECP is washed away, and the bound complex is then incubated with a developing agent.
After stopping the reaction, the fluorescence of the eluate is measured. The fluorescence is directly proportional to the concentration of ECP in the serum sample.