Why are the ImmunoCAP components and ISAC results not in agreement?
Answer: The two methods are based on two different measurement techniques and this may cause discrepancies for some patient samples.
Example: E.g. a peanut sensitized patient would like more information regarding the probability of having a severe reaction to peanut. Molecular testing is performed with the ImmunoCAP and ISAC technologies.
Results: ImmunoCAP Ara h 2 = 4 kU/l, ISAC Ara h 2 < 0.1 ISU
Explanation: Overall similar IgE ab levels are detected with the two technologies for most serum samples [1,2] but discrepant results may occur for individual samples or components. The two technologies differ in binding capacity and detection method; hence the results obtained from the two platforms will differ also theoretically.
ImmunoCAP is a well-established, quantitative technology, while ISAC is a novel methodology and has to be regarded as semi-quantitative. Usually a somewhat higher IgE ab concentration is registered with the ImmunoCAP as compared with the ISAC technology.
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