Phadia MIA

  1. Do I need admin rights to install ISAC Xplain or Phadia MIA on my computer?

    A: Yes, for customers that don’t have Thermo Fisher computers you will need admin rights. B: No, for internal installations within Thermo Fisher you will not need admin rights. It’s the same principal as for IDM. Order your access via IT Support.

  2. The red grid is not visible after scanning ISAC slides, not even if they choose to load images for the recently scanned chips. What is the reason and what can we do?

    Try the following trouble shooting options, one at a time and see if it works. Usually it's enough using "F5". - Refresh the screen with keyboard key F5 - Load several slides and toggle between them - Send me the Scanprotocol.xml file - Verify that the selected image path in MIA exists - As the last attempt, restart the computer.

  3. What PMT value and other adjustments do I need to make after upgrading Phadia MIA at customers?

    We recommend a PMT value of 550 to 600 for scanning. Other adjustments as follows: IgG4/IgG: Limits for the calibration curve: 7.0-8.2, signal level limit for guide dots: 600 IgE: limits for the calibration curve: 5.5-6.6, signal level limit for guide dots: 1000 If intercept values are outside acceptable boundaries, the MIA software will tell you anyway that QC is failed and you will need to adjust PMT until you fall within limits. If intercept is for example below 5.5 in the IgE assay, you will need to increase PMT values, suggested by steps of 20. The basis for calibrating and checking the scanner is the CAL III slide for the LuxScan that is used by the service technicians. This should pass all automatic calibrations and according software QC.

  4. Is it possible that the image automatically can be saved on an external drive instead of the C-drive on the pc? (For back up of data for example)

    The paths to the image folder is set via the “Load Image” button. So; select the external disk via “Load Images” and then select “Scan images” the Image path has then been changed to the external disk. You can see it at the top of the screen. Start a scan and the filed is saved to the external disk.

  5. With the new ISAC 112 slides, a customer had problems getting a full picture of the 4 images, e.g. not all four squares were scanned properly (the grid does not cover the first line). What is the reason and what do I do?

    As the new ImmunoCAP ISAC 112 has a new chip design as well you need to update the ROI (region of interest) for the scanning. To do this you need to select the following coordinates in MIA: Scan Images -> Settings -> IgE -> X = 1400 / Y = 2200 / width = 11000 / height = 37000

  6. Can Phadia MIA 1.1 and 1.2 analyze both ISAC 103 and ISAC 112 slides concurrently?

    Yes, these versions of Phadia MIA will be able to analyze both ISAC 103 and ISAC 112 slides.

  7. Is there a list over the known bugs in the new MIA software?

    Yes, in first hand, please contact ISAC support in this matter but a bug list will be published on DiaNet shortly.

  8. I have difficulties to move a MIA Database from an old Computer with Windows XP to the new one with Windows7 64bit (a Phadia PC). I have local admin rights. How do I solve it?

    The correct order is to changing account of ISACSRV to system user before open the MIA for the first time. This should not be a problem but to be sure do, the following: Remove the default database ImmunoCAPISAC (stop and start server in between) and then restart MIA and let it create the default database. Verify that it is possible to open MIA with the newly created database. Open MSSMSE (NOTE, start the tool by right-click and select “Run as Administrator”) and then select ImmunoCAPISAC right-click and select delete. Click the check box “Close existing connections”. Now put the copied database to a folder on the disk. Attach the database via the MSSMSE tool according to chapter 4 in the technical guide.

  9. What does this error message mean and what can I do about it? “Calibration curve warning Different PMT on calibration and measurement slides. Do you want to proceed anyway?”

    This message means that the latest calibration curve that was run had another PMT value than you have in your scanner at the moment. Probably the PMT value has been “trimmed in” by service personnel after your latest run of calibration curve. Your options are: 1) Run a new calibration curve and proceed with running your samples again (recommended) 2) Click “yes” in the message box and you will get your results. BUT this means that you may get results that are not optimal since they will be measured against the “old” calibration curve that was run with another PMT value.

  10. Why do we experience more flagged red spots with the new software MIA 1.1 and the 112 chip than with the 103 chip?

    ISAC 112 has an improved chip quality, but more spots will be flagged by the new QC method. Users may take this as an indication of decreased ISAC quality, which is not the case. In MIA 3.12, one QC rule had to be fulfilled in order to flag a spot in red. Also, you didn’t need user approval to get the results from the chip. In MIA 1.1 and 1.2, one QC rule is enough to set the QC flag (red marker over spot). If 2 or 3 spots are QC flagged the user must manually set the spot to Good (G), Bad (B) or Negative (N).

  11. What are the QC validation criteria for each spot on the ISAC chip? (When is a spot shown in red?)

    These are the criteria for each spot and triplicate of spots. If one or more of the following criteria are not met, the spot will be marked red a. Spot size must be between 120 and 300 μm b. Signal Mean / Signal median must be between 0.5 and 3 ISU c. Homogeneity within spot must be > 80 % d. Triplicate signal CV must be < 25 % e. Triplicate size CV must be < 25 %

  12. Sometimes we see small white circles positioned for a normal sized spot. We know how to fix this, but why is it a small circle?

    Concerning the feature (white ring) that cannot find the spot correctly: One explanation could be; there is a darker area in the middle of the spot which is slightly bigger than for the other two spots in the triplicate. The algorithm always tries to find the edges when placing the features (white rings) in this case, the derivate can be bigger between areas inside the spot than between the spot edge and the background. We need the original tiff-image to verify this. Meanwhile you have to be observant and move features manually or use the keyboard combination CTRL + A (when the image view is selected), which will run the algorithm once again.

  13. Sometimes we experience dislocated spots in the analyzing step. Why and what do we do about it?

    Dislocated spots should not occur but the spotting procedure is a complex process. Our device works with piezo-technology not getting in contact with the slide as such. If there is a deflection of a single spot, it might not be detected during our internal quality control. MIA should alert in such cases – e.g., giving red circles indicating that customers should check the spot. If it is not clear to which triplicate group the spot belongs I would suggest to flag it as bad. 2 spots are sufficient for getting a positive result.

  14. Why are some spots much smaller than others in the analyzing step for ISAC 112 chip?

    Small spots can be due to the individual behavior of an allergen/antibody and especially the behavior when the binding is supposed to take place. The binding procedure and the reagents are optimized and the surface that the allergens are attached to is the same for all. Often there is nothing wrong with these spots but if they fail one of the QC criteria they will have to be approved manually. The following limitations are present in the software and if one or more criteria of them is not fulfilled you will get a red QC warning. <ul> <li>Spotsize must be between 120 and 300 µm</li> <li>Signal Mean / Signal median must be between 0.5 and 3</li> <li>Homogeneity within spot must be 80%</li> <li>Triplicate signal CV must be &lt; 25%</li> <li>Triplicate size CV must be &lt; 25%</li> </ul> <img style="float: left;" src="/FAQ/Allergy/ImmunoCAP%20ISAC/Small%20spots.jpg" alt="" width="150" height="204" />

  15. This ISAC image has status failed since the guide dots are missing (IgE assay). Is it possible to validate the image anyway?

    <img style="float: left;" src="/FAQ/Allergy/ImmunoCAP%20ISAC/Failed%20assay.jpg" alt="" width="300" height="174" /> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; In this image, the guide dots are negative, which most probably is due to a problem during the detection antibody incubation. There are three possibilities: 1)&nbsp;&nbsp;&nbsp;&nbsp; The detection antibody was not applied sufficiently in the wells 2)&nbsp;&nbsp;&nbsp;&nbsp; The wrong detection antibody was applied (eg. IgG4) 3)&nbsp;&nbsp;&nbsp;&nbsp; The incubation time was not sufficient. The suggestion is to repeat the sample, as you might get false results if the guide dots are missing. If one or two of the guide dots show too weak signal, it might be more possible that the scanner settings are too weak.

  16. Why do we have Non-approved QC in the report as a heading, what does it mean and how do I remove it?

    You can choose if you want to include this heading in the report. If the user during the spot analyzing step sets 2 out of three spots (with the same allergen) or more as BAD (B), these results will be shown under the “Non-approved QC” heading. Please follow the screen shots below for an example. If the customer wants to remove this part of the report, he can go to “EXPORT” (to the left in the application) and remove “Non-approved QC”.

  17. Is it possible to modify the PDF naming convention for ISAC reports?

    The PDF file names are defined in MIA and it is not possible to select the file name. The long file name is set as the following: 8A23224_1326_1_KS11_2011_12_15_13_43_1 Measurement barcode: 8A23224_13:26 (time is only there when measurement barcode already exists in the database) Measurement position: 1 Sample code: KS11 Date: Year_Month_hh_mm_ss The only variable in the file name is the Sample code

  18. How do I choose what headings to show in the report?

    Go to “Export” in the left menu and choose (check-boxes) what parts you want to include in the report. Optional sections are: • Part 1: Summary of positive IgE results • Part 2: ISAC Xplain • Part 3: IgE results sorted by protein group • Part 4: IgE results sorted by allergen source • Part 5: Non-approved QC components

  19. Why can‟t I change the report layout myself after installing the new MIA software 1.1?

    The old MIA software was more flexible and it was possible to change and to customize the report layout. However, now under TFS we need to be consistent with layouts. As for now you are able to use the “Report Editor tool” only in order to translate headings in the MIA reports. If there are special needs for logos, please contact ISAC Support.

As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.