What are the QC validation criteria for each spot on the ISAC chip? (When is a spot shown in red?)

What are the QC validation criteria for each spot on the ISAC chip? (When is a spot shown in red?)
These are the criteria for each spot and triplicate of spots. If one or more of the following criteria are not met, the spot will be marked red a. Spot size must be between 120 and 300 μm b. Signal Mean / Signal median must be between 0.5 and 3 ISU c. Homogeneity within spot must be > 80 % d. Triplicate signal CV must be < 25 % e. Triplicate size CV must be < 25 %
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As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.