What PMT value and other adjustments do I need to make after upgrading Phadia MIA at customers?
We recommend a PMT value of 550 to 600 for scanning. Other adjustments as follows:
IgG4/IgG: Limits for the calibration curve: 7.0-8.2, signal level limit for guide dots: 600
IgE: limits for the calibration curve: 5.5-6.6, signal level limit for guide dots: 1000
If intercept values are outside acceptable boundaries, the MIA software will tell you anyway that QC is failed and you will need to adjust PMT until you fall within limits. If intercept is for example below 5.5 in the IgE assay, you will need to increase PMT values, suggested by steps of 20.
The basis for calibrating and checking the scanner is the CAL III slide for the LuxScan that is used by the service technicians. This should pass all automatic calibrations and according software QC.