1. What PMT value and other adjustments do I need to make after upgrading Phadia MIA at customers?

    We recommend a PMT value of 550 to 600 for scanning. Other adjustments as follows: IgG4/IgG: Limits for the calibration curve: 7.0-8.2, signal level limit for guide dots: 600 IgE: limits for the calibration curve: 5.5-6.6, signal level limit for guide dots: 1000 If intercept values are outside acceptable boundaries, the MIA software will tell you anyway that QC is failed and you will need to adjust PMT until you fall within limits. If intercept is for example below 5.5 in the IgE assay, you will need to increase PMT values, suggested by steps of 20. The basis for calibrating and checking the scanner is the CAL III slide for the LuxScan that is used by the service technicians. This should pass all automatic calibrations and according software QC.

  2. Is it possible that the image automatically can be saved on an external drive instead of the C-drive on the pc? (For back up of data for example)

    The paths to the image folder is set via the “Load Image” button. So; select the external disk via “Load Images” and then select “Scan images” the Image path has then been changed to the external disk. You can see it at the top of the screen. Start a scan and the filed is saved to the external disk.

  3. With the new ISAC 112 slides, a customer had problems getting a full picture of the 4 images, e.g. not all four squares were scanned properly (the grid does not cover the first line). What is the reason and what do I do?

    As the new ImmunoCAP ISAC 112 has a new chip design as well you need to update the ROI (region of interest) for the scanning. To do this you need to select the following coordinates in MIA: Scan Images -> Settings -> IgE -> X = 1400 / Y = 2200 / width = 11000 / height = 37000

As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.