How does the calibration concept for ImmunoCAP ISAC work?
The two calibration samples (Specific IgE/IgG/IgG4 Control sample) for the different methods are the following:
• Specific IgE: CTR02
• Specific IgG/IgG4: KS15
In Phadia MIA 1.1 and 1.2 and earlier versions there is a manual process step to enter what sample on a slide corresponds to a calibration sample.
Criteria for calibration curve:
- Slope is locked to 1.
- Intercept X-axis Specific IgE; 5.5-6.6 (variable)
- Intercept Y-axis Specific IgG/IgG4; 7.0-8.2 (variable)
- Logaritmic model => All allergens have the same impact.
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