This ISAC image has status failed since the guide dots are missing (IgE assay). Is it possible to validate the image anyway?

This ISAC image has status failed since the guide dots are missing (IgE assay). Is it possible to validate the image anyway?
<img style="float: left;" src="/FAQ/Allergy/ImmunoCAP%20ISAC/Failed%20assay.jpg" alt="" width="300" height="174" /> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; In this image, the guide dots are negative, which most probably is due to a problem during the detection antibody incubation. There are three possibilities: 1)&nbsp;&nbsp;&nbsp;&nbsp; The detection antibody was not applied sufficiently in the wells 2)&nbsp;&nbsp;&nbsp;&nbsp; The wrong detection antibody was applied (eg. IgG4) 3)&nbsp;&nbsp;&nbsp;&nbsp; The incubation time was not sufficient. The suggestion is to repeat the sample, as you might get false results if the guide dots are missing. If one or two of the guide dots show too weak signal, it might be more possible that the scanner settings are too weak.
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As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.