Why are there positive IgG spots in one of the corners even though they are running an IgE assay.
These spots are not positive IgG spots, in reality they will always shine either you run an IgE or IgG assay as they are so called guide spots and are needed for the fit of the array over the spots on the chip. The spots called IgG in the array are labeled in the same dye as IgE detection antibody. That’s why they shine in an IgE assay. The corner spots named IgE are labeled with the same dye as for the IgG detection antibody whereas they will shine in an IgG assay.
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