What should the patients be careful about in stool sampling?

What should the patients be careful about in stool sampling? If the samples are rich in mucus, I expect the result of Calprotectin assay tends to increase?

This is one drawback of all calprotectin assays in general, because stool texture is of course important and will influence measured calprotectin levels. For instance very liquid stool might underestimate the calprotectin level, but you can't help it, it is due to the material we work with, an stool is not that standardized as blood. I'm not sure that samples rich in mucus would give higher results, but they may of course differ from samples with normal stool. Because of these limitations calprotectin results should always be interpreted with caution and in the context of stool texture and clinical signs.

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As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.